| 朱明娟① 袁初軍① 阮英茆② 陳穎② 孫仁宇③
①中國醫學科學院中國協和醫科大學血液學研究所（天津，300020，中國）
②中國醫學科學院中國協和醫科大學阜外醫院（北京，100037，中國）
③中國醫學科學院中國協和醫科大學基礎醫學研究所（北京，100730，中國）
摘要 目的 研究中藥腦肺康防治慢性阻塞性肺疾病（COPD）和缺氧性肺動脉高壓（HPH）的療效機理。方法 建立彈性酶、吸烟、細菌感染、缺氧及脂多糖誘發的COPD、肺動脉高壓鼠模型及體外肺泡巨噬細胞、肺泡Ⅱ型上皮細胞培養。采用形態定量病理學、電鏡、病理生理、免疫細胞及分子生物學技術進行檢測。結果 腦肺康對COPD造成的氣道、肺、肺小動脉的病理性結構改建有减輕作用、降低肺動脉高壓；保護肺血管內皮細胞、抑制腫瘤壞死因子（TNF-α）的産生，促進肺泡巨噬細胞發生凋亡；可拮抗TNF-α對肺表面活性物質A-mRNA和B-mRNA表達的抑制。結論 腦肺康對動物實驗性COPD和HPH有明顯的防治作用、其主要機理可能是保護肺血管內皮細胞、抑制肺泡巨噬細胞的産生和誘導型一氧化氮合酶（iNOS）的表達，减少炎症因子。
關鍵詞 腦肺康 慢性阻塞性肺疾病 缺氧性肺動脉高壓
Mechanismic study of the preventive and therapeutic effects of Chinese traditional medicine NAOFEIKANG on chronic obstructive pulmonary disease and hypoxic pulmonary hypertension
ZHU Mingjuan①,YUAN Chujun①, RUAN Yingmao②,CHEN Ying②.
①Institute of hematology,Chinese Academy of Medical Sciences & Peking Union Medical College (Tianjin,300020,China)
②Fuwai hospital,CAMS and PUMC (Beijing,100037,China)
Abstract Objective Mechanismic study of the preventive and therapeutic effects of Chinese traditional medicine NAOFEIKANG on chronic obstructive pulmonary diseases (COPD) and hypoxic pulmonary hypertension (HPH) .methods The aninmal (hemsters or Wistar rats) models of COPD and HPH induced by elastase, smoking,bacteria infective,E Coli Lipopolysaccharide and hypoxia were established. Cell culture of alveolar macrophages and typeⅡ lipneumocytes in Vitro is conducted. The lung samples were observed and measured by morphometric analysis under the olympus light microscope and the electromicroscope,and the pathophysiology,immunohistochemistry and molecularbiology tests were performed. Results Chinese herbs
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